First record of Tomato chlorotic spot virus in the USA

Abstract

A tospovirus was identifed in tomato plants from two counties in Florida by reverse transcription-PCR and sequencing of portions of the S, M and L genomic segments. The predicted amino-acid sequences of the N protein of PCR products from four plant samples were >96% identical to those of TCSV. Partial nucleic acid sequences of the L and M RNA were >97% identical to those reported for TCSV isolates. Extracts from feld samples infected test plants and produced symptoms similar to those reported for TCSV. This is the frst report of an isolate of TCSV in Florida and in the USA. © Brazilian Phytopathological Society.