Corrigendum to: Assessing Symbiodinium diversity in scleractinian corals via next-generation sequencing-based genotyping of the ITS2 rDNA region (Molecular Ecology, (2014), 23, 17, (4418-4433), 10.1111/mec.12869)

Abstract

File S1 ITS2 BLAST database The following changes have been made to the Supplemental File S1: The following sequences have been reverse complemented so that all sequences in the.fasta file run in the same 5′→3′ direction. LJ_A1c LJ_A14 GS_B1i LJ_B23 LJ_B33 LJ_B36 LJ_C3s LJ_C84 LJ_C84a LJ_C86 LJ_C87 LJ_C160 LJ_C161 LJ_C163 LJ_C163a LJ_C163b LJ_D17 Sequences with amplicons that are too short have been deleted. LJ_A1c missing 2 bp 3′ end (deleted) GS_B13 missing 14 bp 5′ end (deleted) ST_B02_71 missing 21 bp 5′ end (deleted) ST_STR1 missing 35 bp 5′ end (deleted) GS_B1k missing 100+ bp 5′ end (deleted) GS_C3_new missing 50 bp 3′ end (deleted) GS_C62(2) missing 50+ bp 3′ end (deleted) GS_C66a missing 29 bp 5′ end (deleted) GS_C125 missing 17 bp 5′ end (deleted) GS_C126 missing 17 bp 5′ end (deleted) LJ_C160 missing 2 bp 3′ end (deleted) LJ_C161 missing 2 bp 3′ end (deleted) LJ_C163 missing 50+ bp 3′ end (deleted) LJ_C163a missing 2bp 3′ end (deleted) LJ_C163b missing 2bp 3′ end (deleted) Sequences with amplicons that are too long have been cropped to the proper length. LJ_A14 2 extra base pairs 5′ end. Base pairs deleted. GS_B1i 10 extra base pairs 3′ end. Base Pairs deleted. LJ_B23 10 extra base pairs 3′ end. Base Pairs deleted. LJ_B33 10 extra base pairs 3′ end. Base Pairs deleted. LJ_B36 10 extra base pairs 3′ end. Base Pairs deleted. LJ_C3s 2 extra base pairs 5′ end. Base Pairs deleted. LJ_C2r 9 extra base pairs 5′ end. Base pairs deleted. LJ_D17 8 extra base pairs 3′ end. Base pairs deleted. ST_FREE(A) 4 extra base pairs 3′ end. Base pairs deleted. ST_B18a_FB4A 14 extra base pairs 3′ end. Base pairs deleted. Sequences with ambiguous nucleotide calls have been deleted GS_F4.1b. Redundant sequence (sequences with identical nucleotide sequences) have been deleted. GS_A1.1 identical to GS_A13. GS_A1.1 deleted. GS_B21 identical to GS_B38. GS_B38 deleted. GS_Cspc identical to GS_C3. GS_Cspc deleted. (C3 sequences verified against SP database) GS_C1ca identical to GS_C1b and GS_C1e. GS_C1ca and GS_C1e deleted. (C1b sequence verified against SP database) GS_C1p identical to GS_C1.8. GS_C1p deleted as it does not match the SP database and C1.8 is not in the SP database. GS_C3d identical to GS_C21. GS_C3d deleted. (GS_C21 verified against SP database) GS_C26 identical to GS_C26a and GS_C35a_(type_2). GS_C26a and GS_C35a_(type_2) deleted. (C21 sequence verified against the SP database) GS_C26.b1 identical to GS_C35_(type_2). GS_C35_(type_2) deleted. GS_C27 identical to GS_C30_(type_1). GS_C30_(type_1) deleted. GS_C31d identical to GS_C30_(type_2). CS_C30_(type2) deleted. GS_C33_(type_1) identical to GS_C33a. GS_C33_(type_1) deleted. GS_C35_(type_1) identical to GS_C35a_(type_1). GS_C35a_(type_1) deleted. GS_C42_(type_1) identical to GS_C42a. GS_C42_(type_1) deleted. GS_C82 identical to GS_C82b. GS_C82b deleted. GS_C113 identical to GS_C114. GS_C114 deleted. GS_F5.2 identical to GS_F5.2a. GS_F5.2a deleted. Key sequences (C1, C3, C15, D1, D4, D6 and A1) from the literature have been checked. There is a D1a and a D4 sequence in the fasta. This is an error as the D1a sequence should be the same as the D4 sequence. This sequence is currently used to identify key clade D Symbiodiniaceae species such as Durusdinium trenchii. The D4 sequence that is currently found in the.fasta has an erroneous indel of a single guanine at position 94. This has been removed so that the sequence is correct. The D1a sequence (now identical to the D4 sequence) has been deleted. All other key sequences have been verified as correct. Prefixes (e.g. ‘LJ_’) to sequence names have been removed.