Screening of lipid degrading microorganisms for wastewater treatment

Abstract

Aims: Fats, oils and greases (FOG) are poorly removable materials in wastewater treatment systems. The aim of this work is to find the most suitable strain(s) for a biological treatment technology of FOGs polluted wastewaters. Methodology and results: The 142 microorganisms from polluted environment were screened for lipase activity (LA) by sequentially using assays on agar-Tween 80, agar- fats, and turbidimetrically measuring the quantity of calcium salts with fatty acids. The isolates G23, G30, and Zb32 showed highest units of LA and were identified by sequence analysis of 16S rRNA genes. Lipid masses were determined gravimetrically after chloroform/ethyl alcohol extraction. In the model solutions with animal fats the strain Pseudomonas aeruginosa G23 reduced mass fractions of mutton fat, beef tallow, and lard by 79±5%, 88±4%, and 80±6% respectively. Under the same conditions Aeromonas punctata G30 reduced: 65±3%, 60±8%, and 75±4%, and P. aeruginosa Zb32 reduced: 47±5%, 52±6% and 73±7%. In the model solutions with FOGs trap specimens as a carbon source from the local cafeteria the strains P. aeruginosa G23, A. punctata G30, and P. aeruginosa Zb32 reduced a lipid mass fraction by 61.5±7%, 45.2±5%, and 37.5±3% respectively. Conclusion, significance and impact of study: The strain P. aeruginosa G23 is the most effective lipid-degrading microorganism and the best candidate to use in biological treatment technology of FOGs polluted wastewater in Kazakhstan.