In vitro amphotericin B susceptibility of Malassezia pachydermatis determined by the CLSI broth microdilution method and etest using lipid-enriched media

Abstract

We determined the in vitro amphotericin B susceptibility of 60 Malassezia pachydermatis isolates by the CLSI broth microdilution method and the Etest using lipid-enriched media. All isolates were susceptible at MICs of ≤1 μg/ml, confirming the high activity of amphotericin B against this yeast species. Overall, the essential agreement between the tested methods was high (80% and 96.7% after 48 h and 72 h, respectively), and all discrepancies were regarded as nonsubstantial. Copyright © 2014, American Society for Microbiology. All Rights Reserved.