MiR-203-3p participates in the suppression of diabetes-associated osteogenesis in the jaw bone through targeting Smad

Abstract

Certain microRNAs (miRs) have important roles in the maintenance of bone development and metabolism, and a variety of miRs are known to be deregulated in diabetes. The present study investigated the role of miR-203-3p in the regulation of bone loss by assessing jaw bones of a rat model of type 2 diabetes. The results indicated that miR-203-3p inhibited osteogenesis in the jaws of diabetic rats and in rat bone marrow mesenchymal stem cells cultured in high-glucose medium. A luciferase reporter assay was used to verify the bioinformatics prediction that miR-203-3p targets the 3'-untranslated region of Smad1, which is an important mediator of the bone morphogenetic protein (BMP)/Smad pathway. Overexpression of Smad1 attenuated the miR-203-3p-mediated suppression of osteogenic differentiation. It was therefore indicated that the BMP/Smad pathway is attenuated and the transforming growth factor-β/activin pathway is promoted by Smad1 reduction. Taken together, it was indicated that miR-203-3p inhibits osteogenesis in jaw bones of diabetic rats by targeting Smad1 to inhibit the BMP/Smad pathway.