Combined Protective Effects of Quercetin, Rutin, and Gallic Acid against Cadmium-Induced Testicular Damages in Young-Adult Rats

Abstract

Cadmium (Cd) is a toxic metal that damages several tissues of animals and humans including the testis. The ameliorative effects of quercetin (QUE), rutin (RUT), and gallic acid (GAL) at 20 mg kg-1 body weight alone or in combination against testicular injury induced by Cd (24 mg kg-1 body weight) in male Wistar rats were evaluated in this study. Forty-two (42) rats were randomly grouped into six (6) groups: (1) vehicle control group, (2) Cd group, (3) RUT+Cd group, (4) GAL+Cd group, (5) QUE+Cd group, and (6) RUT+GAL+QUE+Cd group. At the end of the oral gavage of the tested chemicals, the rats were sacrificed, blood samples were collected, and testes were harvested and processed for biochemical assays. Cd exposure damaged the testis (smaller epithelium thickness and spermatogenesis index and sloughing of the epithelium); increased lipid peroxidation, glutathione S-transferase activity, and DNA fragmentation; and diminished glutathione reductase activity and serum testosterone level 40 days posttreatment. Treatment with the phenolics separately or in combination attenuated the effect of Cd on serum testosterone, glutathione reductase and glutathione S-transferase activities, lipid peroxidation, and percent fragmented DNA. The increased nitric oxide concentration in the QUE+Cd group was attenuated to control values in the combined (RUT+GAL+QUE+Cd) exposure group. Coadministration of the phenolics appears to have more substantial protective effects than their single effects against Cd-induced testicular DNA damage, glutathione S-transferase activity, and the recovery of testosterone levels and spermatogenesis index. Overall, the tested phenolics can reduce testicular damage more efficiently in their combined form than individual administration.