Abstract
The present work established an efficient staining method for fluorescence activated cell sorting (FACS) with Chlorococcum littorale maintaining cellular viability. The method was designed to detect high-lipid cells and to guarantee cellular viability. BODIPY505/515 (BP) was more suitable to FACS when compared to Nile red. The optimum concentrations were 0.4μgml-1 of BP, 0.1% DMSO or 0.35% ethanol. Both ethanol and DMSO were equally efficient and assured cellular viability after the staining and sorting. Here a method is presented to rapidly screen and sort lipid rich cells of C. littorale with FACS, which can be used to produce new inoculum with increased cellular lipid content.
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Cabanelas, I. T. D., van der Zwart, M., Kleinegris, D. M. M., Barbosa, M. J., & Wijffels, R. H. (2015). Rapid method to screen and sort lipid accumulating microalgae. Bioresource Technology, 184, 47–52. https://doi.org/10.1016/j.biortech.2014.10.057
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