Description and role in proliferation of iberiotoxin-sensitive currents in different human mammary epithelial normal and cancerous cells

Abstract

Several studies suggested that potassium channels are involved in the proliferation of cancer cells but the involvement of the large conductance Ca 2+-activated K + channels (BK Ca) in the cancerous phenomenon is still controversial. In the present study, we used iberiotoxin, a specific blocker of BK Ca, and report the activity of an iberiotoxin-sensitive current in various human breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-468 and MDA-MB-435s) as well as in normal mammary epithelial cells (HME). Iberiotoxin and NS1619, an activator of BK Ca, did not interfere with either cell proliferation or with the invasive properties of the cells, under normal culture conditions. However, extracellular pulses of ATP, which induced transient increases in intracellular Ca 2+ concentration, revealed a significant reduction effect of iberiotoxin on cell proliferation. We conclude that the iberiotoxin-sensitive current is not involved in cell proliferation in basal conditions but participates when the intracellular Ca 2+ concentration is increased. These experiments also suggest that BK Ca channels are not involved in the cancerous transformation and are probably a relic from normal cells. © 2004 Elsevier B.V. All rights reserved.