DNA-dependent protein kinase regulates DNA end resection in concert with Mre11-Rad50-Nbs1 (MRN) and Ataxia Telangiectasia-mutated (ATM)

Abstract

Background: The processing of DNA double strand breaks is critical for homologous recombination. Results: The nonhomologous end joining factor DNA-dependent protein kinase (DNA-PK) blocks end resection but is regulated by autophosphorylation, the Mre11-Rad5-Nbs1 (MRN) complex, and the ataxia telangiectasia-mutated (ATM) kinase. Conclusion: DNA-PK regulates DNA end processing for homologous recombination. Significance: Analyzing the effects of NHEJ factors on end processing is essential to our understanding of homologous recombination. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.