Study of Some Enteropathogens Causing Acute Diarrhea in Infants and Children Less than 5 Years Old

Abstract

Background: Diarrheal disorders are a major health issue in pediatrics all over the world. Objectives: To isolate enteropathogens causing diarrhea and determine antibiotic sensitivity pattern. Methodology: 120 stool samples underwent the following procedures; macroscopic and microscopic examination, preparation of wet smear, detection of fecal leucocytes,carbol-fuchsin staining for detection of Campylobacter, Rota virus detection by immunochromatography, culture of samples on Mackongey agar, XLD, API for lactose non fermenting colonies, Identification of EPEC by polyvalent antisera, O157:H7 E.coli detection using Sorbitol Mackoncey and the isolates obtained were tested for antimicrobial sensitivity. Results: The detected enteropathogens were EPEC (46.6%), Rota Virus (20.8%), Salmonella (14.2%), Shigella (1.7%), K.oxytoca (8.3%)and Pseudomonas (4.2%).The antibiotic resistance pattern was ESBL; EPEC (53%), Salmonella (47%). ESBL and Ampc; EPEC (65%), Shigella (5%) and K.oxytoca (25%). Ampc resistance was EPEC while all Pseudomonas isolates were wild. Conclusions: Rehydration therapy, Rota virus vaccination and antibiotic policy are essential for management of diarrheal cases.