Arsenic (As) removal using Talaromyces sp. KM-31 isolated from As-contaminated mine soil

Abstract

Bioremediation is an environmentally-benign and cost-effective approach to removing arsenic from contaminated areas. A fungal strain hyper-tolerant to arsenic was isolated from soil from a mine site and used for the removal of arsenic. The isolated fungus was identified as Talaromyces sp., and its growth rate, arsenic tolerance, and removal rates were investigated for As(III) and As(V). Arsenic tolerance tests revealed that the fungus was highly resistant to arsenic, tolerating concentrations up to 1000 mg/L. Robust mycelial growth was observed in potato dextrose broth containing either As(III) or As(V), and there was no difference in growth between that in arsenic-free medium and medium amended with up to 300 mg/L of either arsenic species. The isolate showed relatively low growth rates at As(V) concentrations >500 mg/L, and almost no growth at As(III) concentrations >300 mg/L. Both arsenic species were effectively removed from aqueous medium (>70%) in tests of the biosorption of arsenic onto mycelial biomass. Surface modification of the biomass with Fe(III) (hydr)oxides significantly enhanced arsenic removal efficiency. The findings indicate that this soil fungal strain has promise for use in bioremediation strategies to remove arsenic from highly contaminated aqueous systems.