Inhibitory mechanism in vitro: Potential of bacterial consortium against shallot wilt disease caused by Fusarium oxysporum

Abstract

This study aimed to examine inhibitory effect of bacterial consortium formulated as bio-activator against wilt disease of shallot caused by Fusarium oxysporum. The study was split into three activities; bio activator preparation, isolation of Fusarium oxysporum and inhibitory test on solid and liquid culture. Pseudomonas flourescens, Actinomycetes sp. and Streptomyces sp. were obtained from collection of Biotechnology Laboratory and prepared as bio-activator in liquid form while F. oxysporum was collected from infected tissue at a highland in Enrekang district. The findings suggest that 2.0% and 2.5% per mL concentration of bio-activator shown to have a much greater rate of limiting filamentous growth of F. oxysporum in both medium. No different in inhibitory distance among trials except control, and a declined trend of all trials and control from time to time occurred. Bacteria with 2.5% concentration was consistent to have a much more considerable pressure of conidium development of F. oxysporum.