Transcriptional profiles of immature ears and tassels in maize at early stage of water stress

Abstract

In the reproductive organs of maize (Zea mays L.), the changes in transcription that occur during meiosis at early stage of water deficit were characterized. We used oligo microarray analysis, which included 57452 transcripts representing more than 30 000 genes, and combined this with reverse Northern blot analysis. After 1 d stress, 1 809 transcripts were differentially expressed (by 2-fold or greater) with 34 % of them being upregulated in the tassels, while in the ears 861 transcripts were differentially expressed with 41 % being upregulated. Of these, 33 transcripts were upregulated in both organs, including those involved in protective functions, reactive oxygen species detoxification, nitrogen metabolism and gibberellin metabolism. In contrast, the transcripts involved in polyamine and sugar metabolism were downregulated. © 2008 Springer Science+Business Media B.V.