Coherence between cellular responses and in vitro splicing inhibition for the anti-tumor drug pladienolide b and its analogs

Abstract

Background: Pladienolide B is a complex natural product that potently inhibits pre-mRNA splicing. Results: The same molecular features of pladienolide B are required for the drug's effects on cell growth, morphology, and splicing. Conclusion: Simplified synthesis and modification of active pladienolide B is possible. Significance: Pladienolide B analogs can be used to study the relationship between splicing and cancer cell function. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.