Purification of the G-protein G13 from rat brain membranes

Abstract

Significant amounts of G13, a member of the recently described G12-subfamily of heterotrimeric G-proteins, have been detected in rat brain membranes by specific antisera. The α-subunits of G13 (Gα13) were purified by using a combination of conventional and subunit-exchange chromatography. Purification was facilitated by the fact that the initial anion-exchange chromatography separated G13 from most of the other G-proteins, including G(q/11). Moreover, Gα13-enriched fractions obtained from this chromatographic step were devoid of βγ-dimers, despite the absence of G-protein-activating agents. Nevertheless, the purified Gα13 retained its ability to interact with βγ-dimers under appropriate conditions, i.e. the addition of Lubrol PX instead of cholate as detergent and the omission of ethylene glycol routinely used as a protecting additive. The interaction was demonstrated by (i) the binding of Gα13 to immobilized βγ-complexes and (ii) the formation of stable heterotrimers during sucrose-density-gradient centrifugation. Furthermore, our studies on Gα13 provide evidence for an extremely slow basal GDP/GTP exchange rate. The purified protein showed negligible binding of guanosine 5'-[γ-[35S]thio]triphosphate (GTP[35S]). Accordingly, dissociation of Gα13 from immobilized βγ-complexes was achieved by AlF4-/Mg2+, but not by GTP[S]. These data indicate that G13 exhibits properties highly distinct from those of other G-proteins.