Functional thermal lens microscopes for ultrasensitive analysis of non-fluorescent molecules and microchip chemistry

Abstract

Thermal lens microscope (TLM) is a kind of absorption spectrophotometry based on photothermal phenomena of non-fluorescent molecules. TLM has high sensitivity (single molecule concentration in fL detection volume) and wide applicability (non-fluorescent molecules). TLM was successfully applied to detection on microchip in clinical diagnosis, environmental analysis, single cell analysis and so on. The basic function of TLM is concentration determination in microspace. In addition, we have realized various functions on TLM for sensitive chiral analysis, individual nanoparticle counting and in situ flow sensing. In this presentation, we explain these functional TLMs for microchip chemistry.