Endolithic bacterial communities in rock coatings from Kärkevagge, Swedish Lapland

Abstract

Rock coatings in Kärkevagge, Swedish Lapland, are widespread and mineralogically diverse. A preliminary study of the rock coatings revealed higher than expected bacterial diversity for an endolithic environment in the arctic. Using 454 Roche pyrosequencing of the 16S rRNA gene, nine rock coating samples from three different coating mineralogies were sequenced. The three coating types include Fe films of goethite and hematite, sulfate crusts of jarosite and gypsum, and aluminum glazes of basaluminite and alunite. Over 20 000 quality sequences were analyzed, and over 2800 operational taxonomic units were identified. Diversity indices and richness estimates confirmed high levels of diversity, particularly in the sulfate crusts with diversity indices at the level of complex soils. Inferred physiology shows the presence of both heterotrophs and autotrophs, with genera of autotrophic Fe and S metabolisms present in at least 2% of the total for each coating type. The most common phyla included Proteobacteria, Acidobacteria, and Actinobacteria - all common soil taxa. Coatings also showed distinct community structure between coating mineralogies. Given the diversity in coating types found in areas receiving the same chemical and environmental inputs, the distinct microbial communities suggest a biological role in coating development. Bacterial community structures in rock coatings from Swedish Lapland are unique to coating mineralogy.