Determination of Kaempferol in extracts of Fusarium chlamydosporum, an endophyticfungi of Tylophora indica (Asclepeadaceae) and its anti-microbial activity

Abstract

Present study describes the characterization and quantification of Kaempferol from methanolic and aqueous extracts of Fusarium chlamydosporum ,an endophytic fungi isolated from Tylophora indica stem. MTT assay was done to examine its cytotoxicity. The CC50 values in both the extracts were 10mg/ml concentration The methanolic extract also exhibited antibacterial activity against drug resistant strains of Psuedomonas aerugenosa and MRSA with an MIC of 500 µg/ml concentration. The HPTLC analysis exhibited the presence of Kaempferol at 0. 21% in aqueous and 0.24% in methanolic extracts. Presence of Kaempferol in Fusarium chlamydosporum(endophytic fungi) in stem of Tylophora indica and its antibacterial activity are not well documented till date.