Study of genetic diversity on six species of Indonesian Coelogyne spp. Based on ISSR markers

Abstract

Background and Objective: Almost all of Coelogyne species from Indonesia are epiphytic. Some of these are facing the extincion and need to be conserved through plant breeding programs. Unfortunately, there are not many research reports on the genetic diversity of orchids which are substantial for genetic conservation and plant breeding program. The study aimed to identify the genetic diversity of some important species of genus Coelogyne spp., performed using inter simple sequence repeats (ISSR) molecular marker. Materials and Methods: The DNA of six orchid species from the genus of Coleogyne spp. was separated and served as samples in the PCR amplification reaction using 10 ISSR primers. Results: This study found that using six orchid species from the genus of Coelogyne spp. (C. pandurata, C. massangeana, C. mayeriana, C. asperata, C. celebensis and C. rumphii), the ISSR primers yielded as many as 106 amplified fragments which varied in size from 250-3000 bp. Conclusion: Moreover, this study showed that the polymorphic amplification bands reached as high as 98.9% and the similarity coefficient of the six orchid species studied revolved between 0.32-0.70, meaning that the genetic diversity of the orchid species studied was spread out between 0.30-0.68.