On the use of Tétrazolium Salt as a Viability Indicator for Conchocelis Filaments–]!. Injury and Tolerance of Filaments as Affected by Environmental Factors

Abstract

It was iound, as reported in the previous paper, that the gradations in the reduction of TTC by filament-, reflected sharply the intact, injured and dead states. Limits of viability of filaments under various deleterious conditions were determined by using TTC as indicator. -. The rtsuits are summarized as follows: 1) Injurious effect of lO ^M. copper sulfate in sea water becomes manifest within 1 day, 10_6M. after 3 days, and 10~8M. after 5 days; 2) The critical temperature of causing damage after 24 hours of incubation is found at 30° to 32°C.; 3 Injury becomes apparent after 3 hours of desiccation at 6&% relative humidity,. and within 1 to 2 hours at 10%, respectively; 4) By the ultraviolet irradiation (quartz mercury sterilizing lamp), severe injury occurs after 30 minutes, and complete killing after an hour. Further research in extended application of the TTC test method to other useful algae is in progress. © 1956, The Japanese Society of Fisheries Science. All rights reserved.