Determination of the glucose in serum by the FT-IR-ATR method

Abstract

The development of instant determinations of glucose has attracted much attention from the viewpoint of diabetic treatment. We propose a determination by using FT-IR with an attenuated total-reflection method (ATR). However, it is not easy to obtain accurate values of the concentrations of glucose in serums. In order to obtain a high correlation coefficient (R) between the concentrations obtained by the FT-IR-ATR method and those obtained by the enzyme method, we improved the method, for example, by changing the shape of the sample holder, and developed an extraction method of the absorption band from the background by using of a second-ordered differential method. Finally, we obtained R = 0.807, which opened a possibility of using the FT-IR-ATR method for the instant determination of glucose. © 2005 The Japan Society for Analytical Chemistry.