Abstract
In situ hybridization (ISH) associated to immunohistochemistry has become a powerful tool for the examination of gene expression. Application of these techniques in grapevine tissues is limited mainly because of technical difficulties with this plant material. Here we present detailed protocols for ISH and immunohistochemistry, recommended controls and troubleshooting, along with examples of several applications to grapevine tissues.
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Colas, S., Jacquens, L., Manteau, S., Devy, J., Conéjéro, G., Clément, C., … Monti-Dedieu, L. (2010). Expression analysis in grapevine by in situ hybridization and immunohistochemistry. In Methodologies and Results in Grapevine Research (pp. 361–374). Springer Netherlands. https://doi.org/10.1007/978-90-481-9283-0_26
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