Influence of nitrate supply on concentrations and translocation of abscisic acid in barley (Hordeum vulgare)

Abstract

Spring barley (Hordeum vulgare L. cv. Golf) was grown at different nitrate supply rates, controlled by using the relative addition rate technique, in order to elucidate the relationship between nitrate‐N supply and root and shoot levels of abscisic acid (ABA). The plants were maintained as (1) standard cultures where nitrate was supplied at relative addition rates (RAs) of 0.03, 0.09 and 0.18 day−1, and (2) split‐root cultures at RA 0.09 day−1 but with the nitrate distributed between the two root parts in ratios of 100:0, 80:20 and 60:40. Time‐dependent changes in root and shoot concentrations of ABA (determined by radioimmunoassay using a monoclonal antibody) were observed in both standard and split‐root cultures during 12 days of acclimation to the different nitrate regimes. However, the ABA responses were similar at all nitrate supply rates. Further experiments were performed with split‐root cultures where the distribution of nitrate between the two root parts was reversed from 80:20 to 20:80 so that short‐term effects to local perturbations of nitrate supply could be studied without altering whole‐plant N absorption. Transient increases in ABA concentrations (maximum of 25 to 40% after 3 to 4 h) were observed in both subroot parts, as well as in xylem sap and shoot tissue. By pruning the root system it was demonstrated that the change in ABA had its origin in the subroot part receiving the increased nitrate supply (i.e. switched from 20 to 80% of the total nitrate supply). The data indicate that ABA responses are easily transmitted between different organs, including transmission from one set of seminal roots to another via the shoot. The data do not provide any indication that long‐term nitrate supplies or general nitrogen status of barley plants affect, or are otherwise related to, the average tissue ABA concentrations of roots and shoots. Copyright © 1995, Wiley Blackwell. All rights reserved