Fabrication of an oriented Fc-fused lectin microarray through boronate formation

Abstract

A stable, covalent, and highly active protein microarray was created through the formation of cyclic esters between surface boronic acids and the carbohydrate moiety of a fusion protein, Fc-dectin-1 (see picture). A biotin-labeled polysaccharide was used as a probe to investigate the binding activity of the protein. Staining of the product array with streptavidin-Cy3 revealed the effectiveness of this immobilization strategy. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.