Low temperature storage of almond pollen

Abstract

Pollen of the California almond cultivars Nonpareil, Ne Plus Ultra, Sonora, and Peerless was evaluated for viability following storage over 12 months at 4, 0, -20, and -80°C. The proportion of viable pollen exceeded 80% for all cultivars and for all temperatures evaluated after 2 months of storage. Following 12 months of storage at 4°C, germination decreased to 8% for 'Nonpareil', 10% for 'Ne Plus Ultra', 50% for 'Sonora', with no germination observed for 'Peerless'. Storage at sub-freezing temperatures maintained pollen viability above 70% in 'Nonpareil', 'Ne Plus Ultra', and 'Sonora' and above 40% in 'Peerless'. Cultivars differed significantly in their tolerance to low temperature pollen storage. Within cultivars, differences in pollen germination following storage at 0, -20, or -80°C were nonsignificant.