Green Synthesis of Silver Nano Particles from Aspergillus niger (MTCC-961)

Abstract

The silver nanoparticles synthesized biologically have been widely used in medicinal field. The synthesis of silver nanoparticles has been carried out by using the extracellular filtrate of the fungal strain. In this study, silver nanoparticles were synthesized using the fungus Aspergillus niger with an aqueous solution of AgNO3. Synthesized silver nanoparticles (Ag-NPs) were characterized through UV-visible spectrophotometer and Fourier Transform Infrared Spectroscopy (FTIR). Maximum absorbance was observed at 420 nm in visible region corresponding to the Plasmon resonance of silver nanoparticles, thus, confirming their presence. The Fourier transform infrared spectroscopy confirmed the presence of protein as stabilizing agent surrounding the silver nanoparticles. The reduction of silver ions was due to amino groups of proteins and other functional groups in the cell free filtrate of fungi. The reduction of silver ions leads to the formation of stable protein capped silver nanoparticles. The Ag-NPs and Ag-NPs + Chloramphenicol (Ab) possess potential antimicrobial activity against to Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus and Streptococcus sp.