A New Spectrophotometric Method to Determine Vitamin B6 in Pharmaceutical Formation Samples Using a Micelle Form

Abstract

An economic and sensitive method was developed to measure pyridoxine hydrochloride (vitamin B6) in pharmaceutical formation as an ion pair, as depending on the charge transfer reaction with SDS as a surfactant and a suitable analytical reagent (chlorazol black). The parameters that gave optimum reaction conditions, such as the concentrations of chlorozal black, SDS, pH, equilibration temperature, time and effect of salting were studied to obtain a linear calibration curve where the linearity range was found to lie between 1.22 × 10 -3 to 34 × 10 -2 mM, and the detection limit (LOD) 2.56 × 10 -4mM. The method was applied successfully to determine vitamin B6 concentrations in various pharmaceutical samples.