Daya Hambat Ekstrak Biji Ketumbar (Coriandrum Sativum L) Terhadap Pertumbuhan Bakteri Shigella Dysenteriae Metode Cakram

Abstract

ABSTRAK Biji ketumbar (Coriandrum Sativum L) merupakan tanaman yang berasal dari Mediterranean, dan Timur Tengah, masyarakat Indonesia umumnya di manfaat kan sebagai bumbu masak. Biji ketumbar sering juga digunakan untuk penyakit diabetes, diuretic, hypolipidemia, anti-fungal, antibiotic baik jamur maupun bakteri, antioxidan, dan anti-inflamasi. Ekstraksi biji ketumbar dilakukan dengan cara maserasi menggunakan pelarut etanol 70%. Konsentrasi yag digunakan 50%, 75%, dan 100%, dengan control positif (cotrimoxazole 5µg/50µl) serta control negative (aquadest steril). Pengujian dilakukan dua kali pengulangan dengan metode difusi cakram. Skrining fitokimia ekstrak biji ketumbar menunjukan adanya senyawa alkaloid, saponin, tannin, flavonoid, fenolik, Triterpenoid, dan glikosida. Hasil pengujian menunjukan bahwa ekstrak biji ketumbar mampu menghambat petumbuhan bakteri shigella dysenteriae. Ekstrak biji ketumbar yang mampu menghambat pertumbuhan bakteri shigella dysenteriae adalah konsentrasi 100% dan rata-rata diameter 8,7 mm dengan daya hambat tergolong sedang. ABSTRACT Coriander seed (Coriandrum Sativum l.) is a plant that originated in the Mediterranean, and the Middle East, Indonesia society generally in stead right as a condiment Cook. Coriander seed is often also used for diabetes, diuretic, hypolipidemia, anti-fungal, bacterial or yeast either antibiotic, antioxidan, and anti-inflammatory. Extraction of coriander seeds is carried out by means of maceration using solvent ethanol 70%. The current concentration of 50% used, 75%, and 100%, with positive control (cotrimoxazole 5 µ g/50 µ l) and negative control (aquadest sterile). The test is performed twice with disc diffusion method of repetition. Phytochemical screening extracts of coriander seeds showed the presence of compounds alkaloids, saponins, tannins, flavonoids, phenolic, Triterpenoid, and glycosides. The test results showed that extracts of coriander seeds capable of inhibiting growth bacteria shigella dysenteriae. Coriander seed extract which is able to inhibit the growth of bacteria of the shigella dysenteriae is the concentration of 100% and an average of 8.7 mm in diameter with drag power belongs belongs to the medium.