A low-cost media for leptolyngbya (cyanobacteria) strain growth isolated from Indonesia as a potential biofuel feedstock

Abstract

The study of a low cost media for Leptolyngbya growth had been done. Commercial NPK fertilizer was one of the low cost media that commonly used for microalgae growth. The objective of this study aimed to determine the best concentration of commercial NPK fertilizer for Leptolyngbya NBP-7A growth as a potential biofuel feedstock. Leptolyngbya NBP-7A was isolated from Cisolok hot spring, located in Indonesia. In this study, Leptolyngbya NBP-7A was grown in commercial NPK fertilizer with concentration 80 ppm, 240 ppm, and in BBM (Bold Basal's Medium), BBM+NPK 80 ppm as a control. This strain was incubated on 35°C. Observation were made approximately 14 days with 2 sampling for each media. The average of wet weight Leptolyngbya NBP-7A after 14 th day observation, was obtained at NPK 80 ppm was 5 mg/L, NPK 240 ppm was 4.1 mg/L, BBM was 19.75 mg/L, and BBM+NPK 80 ppm was 14.65 mg/L). The results of 14 days observation showed that the best concentration NPK culture media for growing Leptolyngbya NBP-7A was in BBM + NPK 80 ppm. This study showed that commercial NPK fertilizer could be used for Leptolyngbya NBP-7A growth as a potential biofuel feedstock.