Development of urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) measurement method combined with SPE

Abstract

A novel separation method of urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) was developed. The C18 and the strong cation-exchange (SCX) columns were employed to separate urinary 8-OHdG. The major interfering substances were removed by the consecutive processes of the C18 and the SCX columns. This newly developed procedure allows researchers to quantitatively measure urinary 8-OHdG by high performance liquid chromatographyelectrochemical detector (HPLC-ECD) successfully. The newly developed separation method produces the optimized procedure of 8-OHdG measurement, and followed by the evaluation of its basic performance. The optimized measurement was highly reproducible (CV = 2.0-2.9%, n = 10). A correlation was observed between the proposed HPLC-ECD method and the column switching method (r = 0.96).