Purification of bacteriocins using size-exclusion chromatography

Abstract

The bacteriocin purification involves following main steps. a) Extraction of cell-free-supernatant of bacteria; b) Ammonium sulfate precipitation; c) Dialysis; d) Diafiltration using PVP and e) Size-exclusion chromatography. However, depending on the nature of work, the compound could be further analyzed by Reverse-phase HPLC, NMR, mass spectrometry and sequencing.