The S1P 2 Receptor Negatively Regulates Platelet-Derived Growth Factor-Induced Motility and Proliferation

Abstract

Sphingosine-1-phosphate (S1P), a bioactive sphingolipid metabolite, is the ligand for five specific G protein-coupled receptors, named S1P 1 to S1P 5 . In this study, we found that cross-communication between platelet-derived growth factor receptor and S1P 2 serves as a negative damper of PDGF functions. Deletion of the S1P 2 receptor dramatically increased migration of mouse embryonic fibroblasts toward S1P, serum, and PDGF but not fibronectin. This enhanced migration was dependent on expression of S1P 1 and sphingosine kinase 1 (SphK1), the enzyme that produces S1P, as revealed by downregulation of their expression with antisense RNA and small interfering RNA, respectively. Although S1P 2 deletion had no significant effect on tyrosine phosphorylation of the PDGF receptors or activation of extracellular signal-regulated kinase 1/2 or Akt induced by PDGF, it reduced sustained PDGF-dependent p38 phosphorylation and markedly enhanced Rac activation. Surprisingly, S1P 2 -null cells not only exhibited enhanced proliferation but also markedly increased SphK1 expression and activity. Conversely, reintroduction of S1P 2 reduced DNA synthesis and expression of SphK1. Thus, S1P 2 serves as a negative regulator of PDGF-induced migration and proliferation as well as SphK1 expression. Our results suggest that a complex interplay between PDGFR and S1P receptors determines their functions. Copyright © 2005, American Society for Microbiology. All Rights Reserved.