Comparison of Candida sp. Colonies in gargling-volume culture from subject wearers of heat-cured and self-cured acrylic resin removable partial dentures

Abstract

Introduction: Candida is a normal microflora commonly found in subjects wearing denture compared with nonwearing denture. Altogether, removable partial denture (RPD) is manufactured by heat-cured and self-cured acrylic resins. Aim: The aim of this study is to observe the comparison of the colonies of Candida sp. in gargling-volume culture from subject wearers of heat-cured and self-cured acrylic resin RPDs. This study was conducted in the Microbiology Laboratory of Veterinarian Faculty, Syiah Kuala University (Unsyiah). Materials and methods: This is an analytical study with an experimental research design. Subjects were obtained by purposive sampling technique and were willing to fill in the informed consent. The gargling volume of six subjects wearing heat-cured and self-cured acrylic resin RPD was inoculated into the Sabouraud 4% dextrose agar (SDA) and incubated at 37°C for 24 to 48 hours. The colonies of Candida sp. were calculated with colony counter. Results: Only 4 of 6 samples (45% precision) had shown colonic growth of Candida sp. However, the t-test analysis shows that the colonies of Candida sp. displayed no significant difference between subjects wearing heat-cured and self-cured acrylic resins RPD (p > 0.05). Conclusion: It was concluded that there was no difference in the number of colonies of Candida sp. in the subject wearers of heat-cured acrylic resin compared with self-cured acrylic resin RPDs. Clinical significance: Clinically, the results of this study showed differences in the number of colonies of Candida sp. In the subjects of RPD users of heat-cured acrylic resins, they contained fewer colonies of Candida sp. than the subjects of RPD self-cured acrylic resin users.