Potential and phylogenetic of superior bacterial isolates in biogas sludge from anaerobic digestion of palm oil mill effluent

Abstract

The study obtains potential and identifies Superior Bacterial Isolates (SBI), i.e., nitrogen-fixing and phosphate solubilizing from biogas sludge. The potential test was conducted using a Completely Randomized Design (CRD) within three replications, and the means were determined by ANOVA and DMRT at Р< 0.05. The molecular identification of SBI is used by the PCR-16S rRNA sequencing method. This study was conducted from January to August 2020. The result found in the N3 and P7 from biogas sludge was more potential in the availability of total-N and available-P compared to other isolates. It also was identified as similar to Bacillus paramycoides and Bacillus cereus, respectively. This information can be used as a reference that biogas sludge can be used to support soil fertility.