Ribotipificación de aislamientos de Mannheimia haemolytica serotipo 1 obtenidos de exudado nasal de bovinos productores de leche en México

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Abstract

One hundred and six (106) isolates of Mannheimia haemolytica serotype 1 (S1) obtained from nasal exudates of clinically healthy cows (n= 80) and cows diagnosed with pneumonia (n= 26) from two dairy farms located in central and northern Mexico were genetic characterized using a ribotyping technique. DNA was digested by HindIII and a probe containing the rrnB rRNA operon was used for ribotyping. Two ribotyping patterns were identified: Rt1 and Rt2, both with bands of approximate size between 0.78 and 19.70 kb. Rt1 had 11 hybridization bands and Rt2, 13. Ninety-six (96) percent of the isolates (102/106) were grouped into a cluster within Rt1; the similarity value between Rt1, Rt2 and the reference strain was 70 %. There were no differences between Rt isolates from healthy cows or cows with pneumonia. These results show that the majority of the strains were grouped within the Rt1 forming a single cluster, regardless of their geographic origin or the cows' health status.

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Jaramillo-Arango, C. J., Hernández-Castro, R., Campuzano-Ocampo, V. M., Delgado-Sapién, G., Morales-Espinosa, R., Xicohtencatl-Cortés, J., … Trigo-Tavera, F. (2017). Ribotipificación de aislamientos de Mannheimia haemolytica serotipo 1 obtenidos de exudado nasal de bovinos productores de leche en México. Revista Mexicana De Ciencias Pecuarias, 8(2), 219–224. https://doi.org/10.22319/rmcp.v8i2.4447

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