Metallo-β-lactamase-producing Bacteroides species can shield other members of the gut microbiota from antibiotics

Abstract

Antibiotics disrupt the intestinal microbiota, rendering patients vulnerable to colonization by exogenous pathogens. Intermicrobial interactions may attenuate this effect. Incubation with ceftriaxone-resistant, ccrA-positive,β-lactamase-producing Bacteroides strains raised the minimum bactericidal concentration of ceftriaxone required to kill a susceptible Escherichia coli strain (mean change,< 0.25 to 29 mg/liter; P0.009); incubation with ceftriaxone-resistant but non-β-lactamase-producing Bacteroides strains had no effect. The production of β-lactamase by common members of the intestinal microbiota (Bacteroides) can protect susceptible fellow commensals from β-lactams.