Optimized Preparation of Carboxymethyl Chitosan/Sodium Alginate Hemostatic Microcapsule

Abstract

In order to prepare and optimize a novel and efficient hemostatic material, a hemostatic microcapsule was prepared by piercing granulation technique with carboxymethyl chitosan and sodium alginate as substrate materials. Three factors in the process (mass percentage of carboxymethyl chitosan, concentration of calcium chloride and pH value of cross-linking system) were investigated through single-factor experiment to explore the effect of experimental factors on water absorbance of hemostatic microcapsule. On the basis of single-factor experiment, orthogonal experiment was conducted to optimize the preparation process of hemostatic microcapsule. Optimized process conditions were obtained as following: mass percentage of carboxymethyl chitosan 25%, concentration of calcium chloride 3.0% and cross-linking system pH=9.0. Microcapsule with normal morphology, uniform particle size, good dispersibility and porous structure was prepared under these conditions. And its water absorbance ratio reached 16.17. In vitro procoagulant test showed that the microcapsule had satisfactory procoagulant effect and was in a dose dependent manner.