Molecular diversity and phylogenetic analysis of eight dromedary camel breeds of Pakistan based on mitochondrial ATP6 and ATP8 genes

Abstract

This work was designed to detect genetic polymorphism in mitochondrial ATP6 and ATP8 genes in eight Pakistani camel breeds (Camelus dromedarius) including Mareecha, Barela, Kachi, Kharani, Thari, Pahari, Watni and Mix-bred (non-descriptive). The genetic relationships among the selected breeds belonging to miscellaneous geographical regions were also carried out through sequences of selected genes. A total of 842 bp including full CDS of both ATP6 and ATP8 genes showed 11 polymorphic sites in all the selected camel breeds. Five polymorphic sites were single variable and six were found parsimony informative sites. The neighbor joining phylogenetic tree was constructed through MEGA 6 program. All Pakistani camel breeds were confirmed as dromedaries. The phylogenetic tree with 23 other mammalian species reconfirmed the classical biological classification. To the best of our knowledge this is the earliest report on selected genes in Pakistani dromedary camel breeds.