Chemical analysis and cytotoxic activity of N-hexane fraction of Zanthoxylum acanthopodium DC. fruits

Abstract

Zanthoxylum acanthopodium DC. the fruit is a potential anticancer agent. This study was evaluated the anticancer activity of n-hexane fraction (nHF) of Zanthoxylum acanthopodium DC. fruit towards T47D cell line. nHFwere analyzed for chemical constituents and tested for cytotoxicity, cell cycle inhibition, apoptosis induction and inhibition of cyclin D1 expression. nHF was found to contain monoterpenes and sesquiterpenes with geranyl acetate (26.34%) as major compound. nHF of Zanthoxylum acanthopodium DC. Fruits were found to have IC50 85.41 ± 0.78 µg/mL, cause accumulation in G0-G1phase, increased apoptosis and decreased cyclin D1 expression. The results reveal that nHF of Zanthoxylum acanthopodium DC. fruits have cytotoxic activities by inhibition cell cycle and induction apoptosis. Our further study is to isolate compounds that responsible for the cytotoxic activity.