Oxidação de proteínas por oxigênio singlete: Mecanismos de dano, estratégias para detecção e implicações biológicas

Abstract

Proteins are potential targets for singlet molecular oxygen ( 1O2) oxidation. Damages occur only at tryptophan, tyrosine, histidine, methionine, and cysteine residues at physiological pH, generating oxidized compounds such as hydroperoxides. Therefore, it is important to understand the mechanisms by which 1O2, hydroperoxides and other oxidized products can trigger further damage. The improvement and development of new tools, such as clean sources of 1O2 and isotopic labeling approaches in association with HPLC/mass spectrometry detection will allow one to elucidate mechanistic features involving 1O2-mediated protein oxidation.