Electrochemical and spectroscopic studies on the interaction between DNA and the product of enzyme-catalyzed reaction of OPD-H2O 2-HRP

Abstract

The pure product of 2,3-diaminophenazine was prepared by the enzyme-catalyzed reaction of o-phenylenediamine-H2O 2-horseradish peroxidase and characterized by UV/Vis spectroscopy, IR spectroscopy and NMR spectroscopy. The electrochemical behaviour of 2,3-diaminophenazine on the glassy carbon electrode was studied. The interaction between 2,3-diaminophenazine and deoxyribonucleic acid was studied by cyclic voltammetry method and UV/Vis spectroscopy, which indicated that the interaction between them is intercalation. The influence of reacting time was also studied. The binding ratio of the 2,3-diaminophenazine-DNA complex is calculated to be 1: 2 and the binding constant is to be 5.07 × 103 L·mol-1 at room temperature.