Mixed antigen ELISA of Mycoplasma pulmonis and M. arthritidis for diagnosis of murine mycoplasmosis

Abstract

Infection of rodent colonies with microorganisms of the genus Mycoplasma may interfere with the results of scientific research. Mycoplasma pulmonis and Mycoplasma arthritidis are the main agents of this genus, and their prevalence can reach 100% in conventional vivariums in developing countries. The detection methods available on the market usually have limitations. One of the most frequently used tests is the enzyme-linked immunosorbent assay (ELISA), which has high sensitivity and specificity and low cost. This work aimed to develop a mixed antigen ELISA test of M. pulmonis and M. arthritidis and evaluate its performance as an alternative diagnostic method for mycoplasmosis. Sera from 81 mice were tested with M. pulmonis antigen (ELISA-Mp), M. arthritidis (ELISA-Ma), and 1:1 mixed antigen (ELISA-mix): 50.61% of the sera showed positive findings in ELISA-Mp, 44.44% in ELISA-Ma, and 46.91% in ELISA-mix. The ELISA-mix had a sensitivity of 72.00%, specificity of 64.28%, and a positive predictive value of 47.36%. Statistical analysis of the data showed agreement between the ELISA-Mp and the ELISA-mix. The mixed ELISA test developed shows satisfactory sensitivity and specificity and may contribute significantly to the improvement of sanitary standards in laboratory animal facilities and to the development of experimental research.