Experimental Study on Optimization of Culture Medium and Culture Environment of Bacillus Megaterium

Abstract

The medium components and culture conditions were investigated through orthogonal test. The experiment showed the optimum fermentation medium (g/L) was composed of peptone 10, beef paste 6, yeast extract 6, sodium chloride 7, agar 15,PH=8.0; The suitable culture conditions were as follows:culture temperature was 30 °C, rotating speed was 200r/min,liquid level was 60mL/250mL,inoculation volume was 5%. Bring up under the optimal conditions, using tablet coating method, the optimum fermentation time was 48h, the number of viable cells reached the peak 2.52108cfu/mL, at this time.