Evaluation of the Wound Healing Potential of Teucrioside

Abstract

Herbal medicines are being used for primary health care due to their efficacy, safety and less side effects. Teucrium genus is a member of the Lamiaceae family, which is a medicinal plant have been used in traditional medicine, especially for wound healing and inflammatory conditions. Teucroside, 9′-decarboxyrosmarinic acid-4′-O-α-rhamnosyl-(1‴→6‴)-O-β-galactosyl-(1‴→4″)-O-α-rhamnoside is a natural phenolic compund which is isolated and identified from of Teucrium genus. In this study, because of bioactive properties of Teucrioside, it was decided to examine its potential wound healing effect. Wound healing process was investigated by in vitro scratch assay which was an easy, inexpensive and well developed method to measure cell migration. In this context, firstly, cell viability was determined by MTT assay and the results were evaluated to find effective concentration for wound healing. Then the cells were incubated for 48 h with extract with defined concentration. Finally, after 48 hours of incubation with teucroside, the wound healing was calculated as 47%. When the data were compared with untreated control (49%), it was concluded that teucroside had not wound healing potential.