Evaluation of antioxidant efficiency of croton tiglium l. Seeds extracts after incorporating silver nanoparticles

Abstract

THIS extracts STUDY by incorporation aimed to enhance of silver efficiency nanoparticles of the different (Ag-NPs) Croton through tiglium raised (C. up tiglium) cytotoxicity seeds against growth of human colon cancer cells. C. tiglium seeds contain various phytoconstituents e.g., carbohydrates (glycosides), flavonoids, sterols (triterpenes), alkaloids and proteins. The protein was hydrolyzed in the dried aqueous seeds extract into free amino acids, the essential amino acids (20.71%) & nonessential amino acids (79.29%). Furthermore, the mucilage in the dried aqueous seeds extract and the lipoidal contents in the saponifiable matter of the petroleum ether extract were represented by total identified sugars represent ~81.21 % of the total mucilage hydrolyzate. The lipoidal contents in the saponifiable matter of the petroleum ether extract were represented by total saturated fatty acids (13.68%), total monoenoic fatty acids (34.49%), total dienoic fatty acids (45.76%) and total identified fatty acids (93.93%). Moreover, the unsaponifiable matter was represented by saturated hydrocarbons (40.73%), unsaturated hydrocarbons (18.93%), fatty alcohols (10.08%), sterols (10.61%) and total identified compounds (80.35%). In addition, there were four compounds (β sitosterol, α-amyrin, Oleanolic acid and 3-O-β-D-glucopyranosyl-β-sitosterol (daucosterol)) isolated from the petroleum ether extract. Additionally, incorporation of Ag-NPs into the extract caused no toxicity on the experimental animals when administrated orally. It was found that the median lethal dose (LD50) of the ethanolic, petroleum ether and aqueous seeds extract-Ag nanocomposites was about 7.95, 5.2 and 65 ml/Kg, respectively.