New Spectrophotometric Determination of Tenofovir Disoproxil Fumaratein Bulk and Pharmaceutical Dosage Form

Abstract

Two new, selective, and sensitive visible spectrophotometric methods (method A and B) were developed for the detn. of tenofovir disoproxil fumarate in bulk and in pharmaceutical prepns. Method 'A' is based on the reaction with wool fast blue, in the presence of buffer at PH 1.5 to form a colored species with a λmax 590 nm. Method B is based on the reaction with tropaeoline -00 dye under acidic condition with a λmax 410 nm. Beer's law is obeyed in the concn. range of 50 to 250 μg/mL for both the methods. The methods were extended to pharmaceutical formulations and there was no interference form any common pharmaceutical excipients and diluents. The result of anal. was validated statistically and by recovery studies. [on SciFinder(R)]