A rat cerebellar protein containing the cdc10/SWI6 motif

Abstract

Systematic analysis of soluble proteins in developing rat cerebellum by an automated two‐dimensional liquid‐chromatography system detected a number of proteins which increased transiently during the initial stage of postnatal development. One of the proteins, V‐1 was isolated using a liquid‐chromatography system, and its amino acid sequence was determined by analysis of the purified protein. The sequence showed that the V‐1 protein consisted of 117 amino acids with an acetylated N‐terminus, and has 2.5 internal sequence repeats of 33 amino acids. Computer retrieval of the sequence indicated that the repeated sequences have a structural characteristics of the cdc10/SWI6 motif, which is found in a series of proteins, including those involved in cell‐cycle control and cell‐fate determination in yeast, Drosophila melanogaster and Caenorhabditis elegans. The structure of V‐1, coupled with its controlled expression in early postnatal development, implies a potential role for V‐1 in cerebellar morphogenesis. Copyright © 1992, Wiley Blackwell. All rights reserved