Identification of Animal Species in Meat Broth by Simplex and Multiplex PCR

Abstract

In some countries, some restaurants may use fraudulent substitution in meat product that has less commercial value or undesirable. Effective laboratory tests must be available for prevention of this phenomenon. In this study meat broth sample was successfully used to identify seven animal species in cooked meals by simplex and multiplex PCR using species specific primers for conserved region of mitochondrial DNA (mtDNA). The results showed effective amplification of mtDNA without cross reaction between primers. Whereas existing methods require mincing and homogenization of direct meat samples, this meat broth sampling method can be performed directly and is thus easier to perform. In conclusion, meat broth sample could be used instead of direct meat tissues for species identification of cooked meat in restaurants to control adulteration substitutions of undesirable meat and protection of the customer rights.