The corrinoid from Methanobacterium thermoautotrophicum (Marburg strain): Spectroscopic structure analysis and identification as Coβ‐cyano‐5′‐hydroxybenzimidazolyl‐cobamide (factor III)

Abstract

The corrinoids from Methanobacterium thermoautotrophicum were extracted as the Co‐cyano derivative, which was isolated in crystalline form. A consistent set of spectroscopic data was acquired (ultraviolet/visible, circular dichroic, infrared, fast‐atom‐bombardment mass, 1H‐NMR and 13C‐NMR spectra), which allowed the structural analysis of this complete corrinoid. It was assigned the structure of the Coβ‐cyano‐5′‐hydroxybenzimidazolylcobamide and was identified with Friedrich and Bernhauer's ‘factor III’ by comparison with an authentic sample. Copyright © 1987, Wiley Blackwell. All rights reserved