In vitro antibacterial activities of Syzygium polyanthum leaves extract-nanoparticle against Salmonella typhimurium, Escherchia coli, and Lactobacillus acidophilus

Abstract

A research with six treatments in a completely randomized design was conducted to evaluate the antibacterial activities of Syzygium polyanthum leaves extract (SpLE)-nanoparticles against Salmonella typhimurium FNCC-0050, Escherichia coli FNCC-0091, and Lactobacillus acidophilus FNCC-0051. The disc diffusion method was used to measure antibacterial activities of the bay leaves extract. The sample plates were incubated at room temperature 37 C for 24 hours, and the antibacterial activity was further determined by measuring the inhibition zone diameter manually using a precision ruler. The treatments were: aquades only (SpLE-0), aquades with 50 ppm antibiotic Tetracycline (SpLE-1), aquades with 0.2% chitosan (SpLE-2), aquades with 0.04% Sodium triphosphate (SpLE-3), aquades with 2.0% SpLE (SpLE-4), and aquades with 2.0% SpLE-N (SpLE-5). One-way ANOVA was used to analysese data statistically and if there were significant differences, it was followed by Duncan's new multiple range test. The results showed that there was an inhibition zone in SpLE against S. typhimurium, E. coli, and L. acidophilus (P <0.001). SpLE-N and chitosan inhibited S. typhimurium and E. coli (P<0.001). While Tetracycline inhibited only E. coli (P<0.001). Currently, findings show that S. polyanthum leaf extract was able to inhibit the growth and colonization of several pathogenic bacteria.